| Gene | Snap25 |
| Chromosome | 2 |
| Deletion Start | 136,781,150 |
| Deletion End | 136,756,133 |
| Deletion Size | 25,018 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,756,336 | 136,756,356 | CGCCCAGACTCTTGACTTGTC |
| TUP | 136,756,361 | 136,756,388 | AAGACGTTTAAGAGGGCAGGGTCCTTTA |
| TUR | 136,756,402 | 136,756,426 | ACAACACACTGTAAGACATCCAAAG |
| TDF | 136,780,658 | 136,780,678 | GATCATTCCCTCGACCACCTG |
| TDP | 136,780,680 | 136,780,701 | TGGCTTTCTTCTCTGCCCAGGC |
| TDR | 136,780,708 | 136,780,730 | CAAACAATGCTCCCACTTGTAAC |
PCR Primers
| Primer | Sequence |
| SU | TATGTGTCTGTTCCTGTGAGG |
| SD | TGTTGTCATTCAGCAAACGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15305A-A8 | VGB6 | C57BL/6NTac | |
| 15305A-H12 | VGB6 | C57BL/6NTac | |
