15313   Mybl2     Status: ES cell colonies screened / QC one positive

GeneMybl2
Chromosome2
Deletion Start163,083,374
Deletion End163,054,832
Deletion Size28,543
Genome Build38
MGI IDMGI:101785
NCBI Gene ID17865
BAC ID375K20
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF163,055,621163,055,640CGCCTTTCCACCGTCTTCAG
TUP163,055,641163,055,664ATGGCCGAACCTGGCCTAGGATGA
TUR163,055,677163,055,698CAAGTAAGCAAGCAGGCATCTC
TDF163,082,541163,082,561TGATTTCAGCCAACCAGTGTC
TDP163,082,563163,082,586CACCCAGCTCCTGTGGCTTCACCT
TDR163,082,592163,082,612GCTGTTACCCTCTTTGCTACC

PCR Primers

PrimerSequence
SUTCGTGTCTTGTACGCTTCGC
SDGCACTTCAGGACTCAGAGGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR78 bp++-
TDFTDR72 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15313B-B11VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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