| Gene | Cd40 |
| Chromosome | 2 |
| Deletion Start | 165,071,014 |
| Deletion End | 165,055,711 |
| Deletion Size | 15,304 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CTGGCCTGTTTCCTGTCAC |
| TUP | | | TCCCATTGTGGACAGATGTCTGCCA |
| TUR | | | CCCAACTGGCAAGGAGAAGATAAC |
| TDF | | | AGGATGGACACAGGGATCTTAGG |
| TDP | | | AGCCAACCATGGGCTACTCCAGG |
| TDR | | | TCCGAGTCAGGGTTTCTCTTG |
PCR Primers
| Primer | Sequence |
| SU | AGGAGAGTCCTTAGCCCTGC |
| SD | CCCACCTGCTAGAGTTTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15316A-F7 | VGB6 | C57BL/6NTac | |
| 15316B-E12 | VGB6 | C57BL/6NTac | |
| 15316B-F10 | VGB6 | C57BL/6NTac | |
