| Gene | Ntsr1 |
| Chromosome | 2 |
| Deletion Start | 180,501,126 |
| Deletion End | 180,500,421 |
| Deletion Size | 706 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 180,500,652 | 180,500,670 | GTGGGCAACTCGGTGACAG |
| TUP | 180,500,675 | 180,500,697 | CACTCTAGCGCGGAAGAAGTCGC |
| TUR | 180,500,721 | 180,500,741 | CAGGCTACCCAGGTGGTAATG |
| TDF | 180,500,842 | 180,500,865 | GTGGCTACTATTTCCTGCGAGATG |
| TDP | 180,500,867 | 180,500,891 | CTGCACCTATGCCACAGCCCTCAAT |
| TDR | 180,500,924 | 180,500,944 | GCCTTGAAGGGATGGCAGATG |
PCR Primers
| Primer | Sequence |
| SU | GTGCAGAGAACCAACCACAG |
| SD | TGAGCGCTCTCCATATGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15320A-H2 | VGB6 | C57BL/6NTac | |
| 15320A-G7 | VGB6 | C57BL/6NTac | |
| 15320A-D9 | VGB6 | C57BL/6NTac | |
| 15320A-G6 | VGB6 | C57BL/6NTac | |
| 15320A-F8 | VGB6 | C57BL/6NTac | |
