| Gene | Arfgap1 |
| Chromosome | 2 |
| Deletion Start | 180,981,453 |
| Deletion End | 180,971,058 |
| Deletion Size | 10,396 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 180,971,060 | 180,971,079 | CAGCCCAAGAACCAGGAAAG |
| TUP | 180,971,081 | 180,971,104 | TCTTAAGGAAGTCCGGGCACAGGA |
| TUR | 180,971,126 | 180,971,144 | GACGCTCATCAGCCCAAAG |
| TDF | 180,981,347 | 180,981,364 | GGCTGGGACAACCAGAAC |
| TDP | 180,981,365 | 180,981,388 | TGGTAGGAGCCTAGCACCCTAGCC |
| TDR | 180,981,416 | 180,981,439 | AGGAGGAACAAGAAGGGCAAAGTG |
PCR Primers
| Primer | Sequence |
| SU | CTGGGAGAGAGGAAGTGCAG |
| SD | GCTGTGGACTTAGGTGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15322A-B8 | VGB6 | C57BL/6NTac | |
| 15322A-B9 | VGB6 | C57BL/6NTac | |
| 15322A-C8 | VGB6 | C57BL/6NTac | |
| 15322A-G7 | VGB6 | C57BL/6NTac | |
| 15322A-G12 | VGB6 | C57BL/6NTac | |
