| Gene | Cpa3 |
| Chromosome | 3 |
| Deletion Start | 20,242,168 |
| Deletion End | 20,215,773 |
| Deletion Size | 26,396 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,241,272 | 20,241,291 | ACCCAGAACCAAGCCTGTCC |
| TUP | 20,241,243 | 20,241,270 | TCATTCCAGTCACGCTTCTCTTCAGTCA |
| TUR | 20,241,206 | 20,241,230 | ACTCAGGAACTAGAATAGGGAGAAC |
| TDF | 20,216,219 | 20,216,240 | GATCACCAGTTGGCTAGACAAG |
| TDP | 20,216,196 | 20,216,217 | TGACCAACAGGCCCTGGGCATC |
| TDR | 20,216,162 | 20,216,182 | TGGTGGCTCATGCCTGTAATG |
PCR Primers
| Primer | Sequence |
| SU | CGTTACTGTCTTATCTCTGTG |
| SD | GGCAAATTGAGAAATGCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15323A-C6 | VGB6 | C57BL/6NTac | |
| 15323A-A9 | VGB6 | C57BL/6NTac | |
