| Gene | Kcnmb2 |
| Chromosome | 3 |
| Deletion Start | 32,182,022 |
| Deletion End | 32,156,117 |
| Deletion Size | 25,906 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,156,206 | 32,156,229 | ATGGAACACTGATGGAACAAGATG |
| TUP | 32,156,231 | 32,156,254 | TGGCTGCTCTCCTTGTCACTGTGA |
| TUR | 32,156,269 | 32,156,290 | AAGCCCTCCCTTTCCTAAGTTG |
| TDF | 32,181,795 | 32,181,816 | TCCTGTCCTGTGCTCATTCTTG |
| TDP | 32,181,818 | 32,181,841 | CCCTTCTCTGCAGCGTGTGGACAG |
| TDR | 32,181,850 | 32,181,868 | CATTCAGCAGGGCACACTG |
PCR Primers
| Primer | Sequence |
| SU | AAGGAGGAATGCACATTTCTG |
| SD | CTGTGCTTGCTTTGACAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15325A-A3 | VGB6 | C57BL/6NTac | |
| 15325A-C5 | VGB6 | C57BL/6NTac | |
| 15325A-D3 | VGB6 | C57BL/6NTac | |
| 15325A-B10 | VGB6 | C57BL/6NTac | |
