| Gene | Tm4sf4 |
| Chromosome | 3 |
| Deletion Start | 57,441,180 |
| Deletion End | 57,425,618 |
| Deletion Size | 15,563 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,426,529 | 57,426,548 | GCAATGAGGGCTGTGGGAAG |
| TUP | 57,426,551 | 57,426,576 | ATTTGCGGTGAGTCGTTCTGGGATCA |
| TUR | 57,426,595 | 57,426,613 | GAACAAGCGGGCAGTGAAC |
| TDF | 57,440,440 | 57,440,460 | GCATCCTGCTGTCCCAACTAC |
| TDP | 57,440,462 | 57,440,486 | TGTTGCCTCACTTATGCAGAAGGCG |
| TDR | 57,440,515 | 57,440,534 | ACCAGGCTCCGAAGGCACTC |
PCR Primers
| Primer | Sequence |
| SU | GATCGCACAAAACGTAATCTC |
| SD | TCCAGACACATTAGTCAAACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15328A-B7 | VGB6 | C57BL/6NTac | |
| 15328A-C5 | VGB6 | C57BL/6NTac | |
| 15328A-E2 | VGB6 | C57BL/6NTac | |
| 15328A-G12 | VGB6 | C57BL/6NTac | |
