| Gene | Hcn3 |
| Chromosome | 3 |
| Deletion Start | 89,160,056 |
| Deletion End | 89,147,502 |
| Deletion Size | 12,555 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,159,384 | 89,159,403 | TGGGAACGCTGGAGAAGAAC |
| TUP | 89,159,355 | 89,159,378 | TCAGTCGGGTCCACAGCTCTGTTT |
| TUR | 89,159,330 | 89,159,347 | TTCCGCCTTCAGCACCAC |
| TDF | 89,148,220 | 89,148,239 | GCGTCATGGAGCAGCATTTG |
| TDP | 89,148,193 | 89,148,217 | ACAACACGACAGAGACATGGCTCGT |
| TDR | 89,148,131 | 89,148,149 | TCCCACAGCACTGGCTTTC |
PCR Primers
| Primer | Sequence |
| SU | AGAAGCAAGCTGAGCACTCC |
| SD | ATTGACGGTACCCAGCTACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15333A-G6 | VGB6 | C57BL/6NTac | |
| 15333A-G12 | VGB6 | C57BL/6NTac | |
| 15333A-H4 | VGB6 | C57BL/6NTac | |
| 15333A-B11 | VGB6 | C57BL/6NTac | |
| 15333A-D11 | VGB6 | C57BL/6NTac | |
| 15333A-E5 | VGB6 | C57BL/6NTac | |
