| Gene | Wars2 |
| Chromosome | 3 |
| Deletion Start | 99,187,733 |
| Deletion End | 99,141,102 |
| Deletion Size | 46,632 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,141,647 | 99,141,666 | CGGTGCTCGGTGATCCTTAG |
| TUP | 99,141,671 | 99,141,695 | CTGCATCTACCAGGCCCTGTAACCA |
| TUR | 99,141,721 | 99,141,740 | GCCAGGCAAGCAACACTATG |
| TDF | 99,187,514 | 99,187,534 | CAGCCTACAGGAATCCTCCAC |
| TDP | 99,187,536 | 99,187,562 | TGGGAAATTACCTTGGAGCCATCGAGA |
| TDR | 99,187,595 | 99,187,616 | GGTCCACGATGCTGTATATCAC |
PCR Primers
| Primer | Sequence |
| SU | TTTGCCAGAATCATTGACTGC |
| SD | ATTGGTCACACAATTCACTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15335A-A7 | VGB6 | C57BL/6NTac | |
| 15335A-C8 | VGB6 | C57BL/6NTac | |
