| Gene | Dkk2 |
| Chromosome | 3 |
| Deletion Start | 132,178,204 |
| Deletion End | 132,173,787 |
| Deletion Size | 4,418 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 132,174,284 | 132,174,303 | GCCCACCAAGTACAAAGGAC |
| TUP | 132,174,304 | 132,174,329 | TCTCACCTGAAGCTTGTCTCCACTCA |
| TUR | 132,174,407 | 132,174,431 | TGCTAACCACTTTCTGCAATTTCTC |
| TDF | 132,177,280 | 132,177,303 | GAGTTCCAATCCTCCTCACTTCTG |
| TDP | 132,177,304 | 132,177,329 | TAAATGCTGGGTAGAGCACGGCAGTT |
| TDR | 132,177,348 | 132,177,372 | GGGATCTCCTTATGTCTGTCTTCTG |
PCR Primers
| Primer | Sequence |
| SU | TTACAGCAGGGACCTATTGG |
| SD | TTCCCTGTTCTTCAGCGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 148 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15341A-D5 | VGB6 | C57BL/6NTac | |
| 15341B-D10 | VGB6 | C57BL/6NTac | |
