| Gene | Cnr1 |
| Chromosome | 4 |
| Deletion Start | 33,945,090 |
| Deletion End | 33,943,617 |
| Deletion Size | 1,474 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,944,016 | 33,944,036 | AACCTGCTGGTGCTATGTGTC |
| TUP | 33,944,038 | 33,944,060 | TCCTTCACTCCCGCAGTCTCCGA |
| TUR | 33,944,078 | 33,944,095 | GCCAGGCTGCCAATGAAG |
| TDF | 33,944,744 | 33,944,768 | GACGGTGTTTGCCTTCTGTAGTATG |
| TDP | 33,944,770 | 33,944,791 | TCTGCCTGCTGAACTCCACCGT |
| TDR | 33,944,823 | 33,944,843 | GCGGAAAGCATGTCTCAGGTC |
PCR Primers
| Primer | Sequence |
| SU | TCTTCCCATGAGTTGCACAG |
| SD | CACTGACACACAACTAACAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15346A-A11 | VGB6 | C57BL/6NTac | |
| 15346A-C7 | VGB6 | C57BL/6NTac | |
| 15346A-C9 | VGB6 | C57BL/6NTac | |
| 15346A-D8 | VGB6 | C57BL/6NTac | |
| 15346A-E1 | VGB6 | C57BL/6NTac | |
| 15346A-H4 | VGB6 | C57BL/6NTac | |
