| Gene | Dnaja1 |
| Chromosome | 4 |
| Deletion Start | 40,732,973 |
| Deletion End | 40,723,716 |
| Deletion Size | 9,258 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,724,412 | 40,724,433 | CCTTGGATAATACCACCTGCTC |
| TUP | 40,724,436 | 40,724,460 | AGAACCCAGTGGTAAGTGCAGTGGC |
| TUR | 40,724,477 | 40,724,495 | TCTGCTTGCTCAGGGTTAC |
| TDF | 40,732,350 | 40,732,369 | CACCTGGCCTGGGTGTGATG |
| TDP | 40,732,370 | 40,732,389 | CGTGCCTATAAGCTAGCACT |
| TDR | 40,732,390 | 40,732,411 | ACCCTCCCAATTCTACCTTTCA |
PCR Primers
| Primer | Sequence |
| SU | GTTCGAGTTTACAGCTGAATC |
| SD | CCCATAGCTTAACAATGAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 62 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15347A-A8 | VGB6 | C57BL/6NTac | |
| 15347A-B12 | VGB6 | C57BL/6NTac | |
| 15347A-H5 | VGB6 | C57BL/6NTac | |
| 15347A-D4 | VGB6 | C57BL/6NTac | |
| 15347A-G12 | VGB6 | C57BL/6NTac | |
| 15347A-C6 | VGB6 | C57BL/6NTac | |
