| Gene | Aldob |
| Chromosome | 4 |
| Deletion Start | 49,544,905 |
| Deletion End | 49,536,744 |
| Deletion Size | 8,162 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,544,401 | 49,544,420 | TGTCATGCAAACGGAACTGG |
| TUP | 49,544,369 | 49,544,396 | TGAGCTCTGCCTTATCCTAGTTTGGACC |
| TUR | 49,544,313 | 49,544,336 | AACTTTCTCAGACACAACCCTCAG |
| TDF | 49,537,076 | 49,537,100 | GCCTCAAACCTGACAAACAAATAAC |
| TDP | 49,537,046 | 49,537,075 | CAGAACACTGGGAGAATAAGGGAGAAGACA |
| TDR | 49,537,027 | 49,537,045 | TGTTGGTGTCCCTTCCTTC |
PCR Primers
| Primer | Sequence |
| SU | CAACCTCATGATCAGTGAGC |
| SD | ATGCAGGATCCCTCAACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15350A-A6 | VGB6 | C57BL/6NTac | |
| 15350B-F10 | VGB6 | C57BL/6NTac | |
| 15350B-A9 | VGB6 | C57BL/6NTac | |
| 15350C-G4 | VGB6 | C57BL/6NTac | |
