| Gene | Rnf20 |
| Chromosome | 4 |
| Deletion Start | 49,656,077 |
| Deletion End | 49,633,285 |
| Deletion Size | 22,793 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,633,364 | 49,633,382 | CAGGGACCACCGTGGAAAC |
| TUP | 49,633,392 | 49,633,418 | AGGAGGTGTCTCTTCAACGGTACGAGA |
| TUR | 49,633,435 | 49,633,454 | CTGACAGAAGCCAAGCACAC |
| TDF | 49,655,911 | 49,655,933 | GCGCAAGTGTCCCAAGTGTAATG |
| TDP | 49,655,939 | 49,655,965 | TTTGGTGCCAATGATTTCCATCGCATC |
| TDR | 49,655,988 | 49,656,007 | TGCCTAGCCAGTCCTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | ATGTTTCCCACGTCTCTTGC |
| SD | GGCTTCAAACCTTCCAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15351A-B9 | VGB6 | C57BL/6NTac | |
