| Gene | Tal2 |
| Chromosome | 4 |
| Deletion Start | 53,786,247 |
| Deletion End | 53,785,824 |
| Deletion Size | 424 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,785,857 | 53,785,874 | TGGAGGCAGCAGAGTGTC |
| TUP | 53,785,877 | 53,785,902 | CAATGCCTTTGCCAAGCTGAGGAAAC |
| TUR | 53,785,909 | 53,785,929 | TCTTGTCTGGAGGGTGAGTAG |
| TDF | 53,786,067 | 53,786,085 | CGCCTGCCAGATGAGGATG |
| TDP | 53,786,096 | 53,786,122 | ACTGAATGACTACCGAGTTCCTTCCCC |
| TDR | 53,786,144 | 53,786,164 | TGACTGTCACCACCACCTTAG |
PCR Primers
| Primer | Sequence |
| SU | TGGTCTTTGGCCCTTTCTTC |
| SD | GAGGTCAAAGGAGGCTTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15352A-C3 | VGB6 | C57BL/6NTac | |
| 15352A-E3 | VGB6 | C57BL/6NTac | |
| 15352A-H2 | VGB6 | C57BL/6NTac | |
| 15352A-H10 | VGB6 | C57BL/6NTac | |
