| Gene | Ambp |
| Chromosome | 4 |
| Deletion Start | 63,154,052 |
| Deletion End | 63,143,367 |
| Deletion Size | 10,686 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,153,694 | 63,153,714 | AAGCCTAGTGCCTTCCAAATC |
| TUP | 63,153,673 | 63,153,693 | TGCGCTGTCCTCATGGGTCCC |
| TUR | 63,153,634 | 63,153,653 | TGTCCACCCTATGCCACCTG |
| TDF | 63,143,901 | 63,143,920 | CCCTGGCCCTGTCTATTGTC |
| TDP | 63,143,876 | 63,143,897 | TGCATGTCCCAACAGCAGGTGC |
| TDR | 63,143,835 | 63,143,853 | AGGCCGCTGTGGAGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | GGAGAGTCTTGGGAGGAAGG |
| SD | GGGACTGTTGTGTTTGAAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15353A-D1 | VGB6 | C57BL/6NTac | |
| 15353A-A11 | VGB6 | C57BL/6NTac | |
| 15353A-B7 | VGB6 | C57BL/6NTac | |
| 15353A-G11 | VGB6 | C57BL/6NTac | |
| 15353A-E11 | VGB6 | C57BL/6NTac | |
| 15353A-G3 | VGB6 | C57BL/6NTac | |
