| Gene | Gpx7 |
| Chromosome | 4 |
| Deletion Start | 108,406,674 |
| Deletion End | 108,400,767 |
| Deletion Size | 5,908 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,406,282 | 108,406,300 | GCTCTCCGCTTTGCCTCTC |
| TUP | 108,406,242 | 108,406,271 | AAGAAACCCTAGCCTTTATGGCCCTTTGCC |
| TUR | 108,406,221 | 108,406,240 | GAGCAGAAGCTGGACTGGAC |
| TDF | 108,400,867 | 108,400,889 | CCCGTATTACAGAGCAGGTGATG |
| TDP | 108,400,831 | 108,400,858 | CCTTCGGAAACGAGAAGACTTGTGACCG |
| TDR | 108,400,793 | 108,400,814 | ACTTGAGTTTGCGGAGGGATGG |
PCR Primers
| Primer | Sequence |
| SU | TTCCAGCACTTTCCCGTTAC |
| SD | AAGAATGGCAAACACATTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15361A-G12 | VGB6 | C57BL/6NTac | |
| 15361A-G2 | VGB6 | C57BL/6NTac | |
| 15361A-E2 | VGB6 | C57BL/6NTac | |
| 15361A-F11 | VGB6 | C57BL/6NTac | |
| 15361A-F6 | VGB6 | C57BL/6NTac | |
| 15361A-A10 | VGB6 | C57BL/6NTac | |
