| Gene | Prpf38a |
| Chromosome | 4 |
| Deletion Start | 108,579,111 |
| Deletion End | 108,565,222 |
| Deletion Size | 13,890 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,578,937 | 108,578,956 | GGCTTACGGGTGGTTTCTTC |
| TUP | 108,578,912 | 108,578,934 | TCTGAGTGAGGAAGGACGGACCC |
| TUR | 108,578,852 | 108,578,870 | GTCCAGGCACAGAGCAGTT |
| TDF | 108,565,720 | 108,565,739 | AAGCAGGGACTGTGGAGGAG |
| TDP | 108,565,688 | 108,565,717 | CTGCCTACTGTCTTGCTTTCTTACACAGCC |
| TDR | 108,565,648 | 108,565,669 | CCAGCCCACTGAGGACATTATC |
PCR Primers
| Primer | Sequence |
| SU | CAGTGTGATTCTGATTGGCG |
| SD | GCAGCAGCATAAACCAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15362A-B10 | VGB6 | C57BL/6NTac | |
| 15362A-D7 | VGB6 | C57BL/6NTac | |
| 15362A-G5 | VGB6 | C57BL/6NTac | |
| 15362A-F10 | VGB6 | C57BL/6NTac | |
