| Gene | Gjb3 |
| Chromosome | 4 |
| Deletion Start | 127,326,734 |
| Deletion End | 127,325,832 |
| Deletion Size | 903 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,326,696 | 127,326,719 | CAAGACCTATTGAGTGGCGTGAAC |
| TUP | 127,326,671 | 127,326,694 | AGTACTCCACGGCATTTGGGCGCA |
| TUR | 127,326,631 | 127,326,650 | TACACCAGCACCCGGAAGAC |
| TDF | 127,326,255 | 127,326,272 | TTCACCATGCCGCGTCTG |
| TDP | 127,326,231 | 127,326,253 | TACAGTGCGCCAGCATAGTACCC |
| TDR | 127,326,198 | 127,326,218 | CCGAGCGATGTAGCAATCCAC |
PCR Primers
| Primer | Sequence |
| SU | TTTCTTCTGGACTCTGACATG |
| SD | GCCAAACCTTCTCATGGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15368A-A7 | VGB6 | C57BL/6NTac | |
| 15368A-B12 | VGB6 | C57BL/6NTac | |
| 15368A-C7 | VGB6 | C57BL/6NTac | |
| 15368A-F6 | VGB6 | C57BL/6NTac | |
| 15368A-E8 | VGB6 | C57BL/6NTac | |
