| Gene | Kcnk3 |
| Chromosome | 5 |
| Deletion Start | 30,622,846 |
| Deletion End | 30,588,320 |
| Deletion Size | 34,527 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,589,146 | 30,589,164 | TGAGTTTGGGCTGCACCAG |
| TUP | 30,589,166 | 30,589,190 | CAGGGCCTGTAATTCTCTGAGGGCA |
| TUR | 30,589,217 | 30,589,238 | GGGAAGAAGGCTAACAGGACAG |
| TDF | 30,621,917 | 30,621,936 | GACGGAGGCAAGGTGTTCTG |
| TDP | 30,621,939 | 30,621,961 | TGTTCTACGCGCTGCTGGGCATC |
| TDR | 30,621,993 | 30,622,013 | CGAAGGTGTTGATGCGTTCAC |
PCR Primers
| Primer | Sequence |
| SU | ACGGCTCCCCAGTCCTTAT |
| SD | CCGAGAACATTAGTTCCCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15379A-B3 | VGB6 | C57BL/6NTac | |
| 15379A-D2 | VGB6 | C57BL/6NTac | |
| 15379A-D7 | VGB6 | C57BL/6NTac | |
| 15379A-F9 | VGB6 | C57BL/6NTac | |
| 15379A-H7 | VGB6 | C57BL/6NTac | |
