| Gene | Dnajc5g |
| Chromosome | 5 |
| Deletion Start | 31,111,465 |
| Deletion End | 31,108,893 |
| Deletion Size | 2,573 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,109,351 | 31,109,371 | GTAGACCTTGCCTGCATCTTG |
| TUP | 31,109,390 | 31,109,419 | CCCTTGCATTATCTAAAGACCCAGACCTGC |
| TUR | 31,109,443 | 31,109,463 | GAGGGCTACAGGAAGCAACTG |
| TDF | 31,110,612 | 31,110,635 | AGAAGGATGATCTAGGAGCAGAGC |
| TDP | 31,110,637 | 31,110,660 | TTTGCCTAGCACGTGAAAGGCCCT |
| TDR | 31,110,664 | 31,110,682 | CCGCAGGTTGGGAATGAAC |
PCR Primers
| Primer | Sequence |
| SU | GAGAGGAACGCGAGTCTAGG |
| SD | CATAGCTTCTGAAGCAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15380A-C6 | VGB6 | C57BL/6NTac | |
| 15380A-E6 | VGB6 | C57BL/6NTac | |
| 15380A-F6 | VGB6 | C57BL/6NTac | |
| 15380A-H10 | VGB6 | C57BL/6NTac | |
