| Gene | Corin |
| Chromosome | 5 |
| Deletion Start | 72,504,145 |
| Deletion End | 72,471,857 |
| Deletion Size | 32,289 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,503,242 | 72,503,263 | CAGGCAAGTAGTCCCAGCAATC |
| TUP | 72,503,210 | 72,503,238 | TCTCACTGAAACTTCTCAGAACTTCGTGC |
| TUR | 72,503,155 | 72,503,179 | AAAGCACAGACATGGACATAGAGAC |
| TDF | 72,471,931 | 72,471,951 | CAGAAGCTGGTGACTGCTAAC |
| TDP | 72,471,910 | 72,471,930 | TTGCTGCGCTTCCTCCTGCTG |
| TDR | 72,471,877 | 72,471,895 | CACGATGAGGGCGCAGATG |
PCR Primers
| Primer | Sequence |
| SU | GGGTAAGATCCACCCTCCTC |
| SD | ATACACATATATAGCAGAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15383A-B5 | VGB6 | C57BL/6NTac | |
| 15383A-B11 | VGB6 | C57BL/6NTac | |
| 15383A-C12 | VGB6 | C57BL/6NTac | |
| 15383A-F10 | VGB6 | C57BL/6NTac | |
