| Gene | Txk |
| Chromosome | 5 |
| Deletion Start | 72,736,432 |
| Deletion End | 72,696,484 |
| Deletion Size | 39,949 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,736,334 | 72,736,353 | GCTCTGAGGGCAGTTTCTTG |
| TUP | 72,736,308 | 72,736,332 | AAATGGGTGAACGCAGTTTCCTGGC |
| TUR | 72,736,275 | 72,736,296 | CAAAGCAACAGACACAGACAGG |
| TDF | 72,696,597 | 72,696,619 | ATTTGCTGAGCTGCTTCAGGTTC |
| TDP | 72,696,573 | 72,696,596 | TCACGGAGATCGCAGAAACGTGGT |
| TDR | 72,696,552 | 72,696,572 | GCTGGCACTGCATTTAAGGTC |
PCR Primers
| Primer | Sequence |
| SU | CAGCAGTAGCCACGACAAAG |
| SD | CCTCTTTGCATGGACCACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15384A-C8 | VGB6 | C57BL/6NTac | |
| 15384A-E2 | VGB6 | C57BL/6NTac | |
| 15384A-G4 | VGB6 | C57BL/6NTac | |
| 15384A-G8 | VGB6 | C57BL/6NTac | |
