| Gene | Chic2 |
| Chromosome | 5 |
| Deletion Start | 75,044,295 |
| Deletion End | 75,006,614 |
| Deletion Size | 37,682 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,043,750 | 75,043,768 | CTTTGGGACTCTGCCTGAC |
| TUP | 75,043,718 | 75,043,746 | CAGCCTATTGACATATGGCTCAGAGGTCA |
| TUR | 75,043,691 | 75,043,712 | GCCACTGGGATCTTAACACCTC |
| TDF | 75,007,542 | 75,007,563 | TCAACCTGGAATGGAAGTGTTC |
| TDP | 75,007,514 | 75,007,539 | TCCTGAGGCAAATCAACCCAAGAGGT |
| TDR | 75,007,477 | 75,007,497 | GTCCTGACAAGGGAGGATCTG |
PCR Primers
| Primer | Sequence |
| SU | GTTGTTCCTGCCCACGGTA |
| SD | GTTCCTGGGAAGGTCAGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15386A-A5 | VGB6 | C57BL/6NTac | |
| 15386A-F2 | VGB6 | C57BL/6NTac | |
| 15386A-C4 | VGB6 | C57BL/6NTac | |
| 15386A-G7 | VGB6 | C57BL/6NTac | |
| 15386A-C8 | VGB6 | C57BL/6NTac | |
