| Gene | Ugt2b34 |
| Chromosome | 5 |
| Deletion Start | 86,906,917 |
| Deletion End | 86,891,180 |
| Deletion Size | 15,738 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,863,432 | 86,863,451 | GATCTGGGACTGGTGGGAAG |
| TUP | 86,906,812 | 86,906,836 | CTGGTGTGGCCAATGGAGTTCAGCC |
| TUR | 86,906,774 | 86,906,795 | CAGAAGCTCGTCTAGGATTGTC |
| TDF | 86,891,773 | 86,891,797 | TCTCAAGGTCATCAATGCAATCATC |
| TDP | 86,891,739 | 86,891,766 | TGACAATGAACCACCTCATTTGCAGTGC |
| TDR | 86,891,713 | 86,891,735 | CAGAATATGTTCAGGAGGCGTTC |
PCR Primers
| Primer | Sequence |
| SU | AATGTGACTGGACAGTTTGCC |
| SD | CAGGCTAGTTGGCCTGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 43364 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15387B-C7 | VGB6 | C57BL/6NTac | |
| 15387B-C10 | VGB6 | C57BL/6NTac | |
| 15387C-E7 | VGB6 | C57BL/6NTac | |
| 15387A-D1 | VGB6 | C57BL/6NTac | |
