| Gene | Cxcl15 |
| Chromosome | 5 |
| Deletion Start | 90,801,629 |
| Deletion End | 90,794,574 |
| Deletion Size | 7,056 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,795,121 | 90,795,144 | TGGCTCCTAAGTCAAAGTAAAGAC |
| TUP | 90,795,146 | 90,795,172 | TGAGCACTAGGGACTTGCTCTTCATGA |
| TUR | 90,795,193 | 90,795,212 | GTCACAGGGACGGACTGTAG |
| TDF | 90,801,342 | 90,801,363 | TTGGGAGACCTGAGAACAAGAG |
| TDP | 90,801,369 | 90,801,395 | TTCCCTTTCCAATTCGGGAGACCTCTA |
| TDR | 90,801,452 | 90,801,473 | CACTGCCTGTCAAGCTGACTTC |
PCR Primers
| Primer | Sequence |
| SU | AAATTATGTTCTCGTGGCCC |
| SD | ACTGAAGACAAGGAGCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 132 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15389A-D9 | VGB6 | C57BL/6NTac | |
| 15389B-C2 | VGB6 | C57BL/6NTac | |
| 15389C-C9 | VGB6 | C57BL/6NTac | |
| 15389C-G2 | VGB6 | C57BL/6NTac | |
