| Gene | Fam47e |
| Chromosome | 5 |
| Deletion Start | 92,590,616 |
| Deletion End | 92,585,286 |
| Deletion Size | 5,331 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,585,537 | 92,585,560 | GTGGAGAGGATGTGATAAGCAAGG |
| TUP | 92,585,562 | 92,585,591 | TTGAATTCTGCCTTCATGGTAAACACGGTA |
| TUR | 92,585,595 | 92,585,616 | AGGAGCGATGTCCTGTCTTTAG |
| TDF | 92,590,373 | 92,590,396 | CTGCTTCTCACTCAAGTTCTCTAC |
| TDP | 92,590,402 | 92,590,431 | AGGTCTAGCCTAAGGAAATCTGCCAGAGGA |
| TDR | 92,590,434 | 92,590,455 | GCCCTCCTGCTCTAATAAACAC |
PCR Primers
| Primer | Sequence |
| SU | TTGTCACTCTTGGGTGAAATATG |
| SD | ACTACAAAAAAACCCTAAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15390B-H7 | VGB6 | C57BL/6NTac | |
| 15390C-G4 | VGB6 | C57BL/6NTac | |
| 15390C-G8 | VGB6 | C57BL/6NTac | |
