| Gene | Kctd10 |
| Chromosome | 5 |
| Deletion Start | 114,380,425 |
| Deletion End | 114,365,588 |
| Deletion Size | 14,838 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,379,873 | 114,379,896 | GCACCTGGATACCTACCTAATCAC |
| TUP | 114,379,844 | 114,379,867 | TTGAGGCCCTAGGTGACCACACCT |
| TUR | 114,379,802 | 114,379,823 | CCCATAACGCAGACAGCAACAG |
| TDF | 114,366,490 | 114,366,511 | GAGGCTTTGGGAGATGTGGTTC |
| TDP | 114,366,467 | 114,366,489 | CATGGGATCCTGGAGCTGGCAAC |
| TDR | 114,366,413 | 114,366,432 | TGAGGTGGCTCAGAGGGTAG |
PCR Primers
| Primer | Sequence |
| SU | TGGGACTAAACGCCTGACTC |
| SD | AGGAGAGCAAAGACTGCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15394A-C8 | VGB6 | C57BL/6NTac | |
| 15394A-G12 | VGB6 | C57BL/6NTac | |
