| Gene | Mdh2 |
| Chromosome | 5 |
| Deletion Start | 135,790,284 |
| Deletion End | 135,778,739 |
| Deletion Size | 11,546 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 135,779,215 | 135,779,237 | CCAGGGATTGCTTGCTTTATGTC |
| TUP | 135,779,238 | 135,779,260 | CGTTTATGTGGACCAGGCTGGCC |
| TUR | 135,779,279 | 135,779,297 | CCGGTACTGAGGCAGAGAC |
| TDF | 135,789,988 | 135,790,007 | AGTGAGGGACTGGCCGTGTG |
| TDP | 135,790,017 | 135,790,038 | AGCTCTGCGCTGCCAAGCTAGA |
| TDR | 135,790,053 | 135,790,076 | CTGAATGAGAAGGGAAACGGTGAC |
PCR Primers
| Primer | Sequence |
| SU | GCCCAGAGATGACTTCTTTG |
| SD | CTGCCTAACGTGGGAATAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15396A-A11 | VGB6 | C57BL/6NTac | |
| 15396A-B2 | VGB6 | C57BL/6NTac | |
| 15396A-G6 | VGB6 | C57BL/6NTac | |
| 15396A-C6 | VGB6 | C57BL/6NTac | |
| 15396A-E6 | VGB6 | C57BL/6NTac | |
| 15396A-E10 | VGB6 | C57BL/6NTac | |
