| Gene | Ptcd1 |
| Chromosome | 5 |
| Deletion Start | 145,165,504 |
| Deletion End | 145,148,285 |
| Deletion Size | 17,220 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 145,165,156 | 145,165,176 | AGCTCAAGGAACAGGGTGGTG |
| TUP | 145,165,129 | 145,165,152 | AGGAAGAACTGGAGCCCAGACCGT |
| TUR | 145,165,075 | 145,165,097 | TCAGACGTTTGCACTGAAAGAAG |
| TDF | 145,148,982 | 145,149,001 | TGGTGCCCGTTACCTGTTTC |
| TDP | 145,148,954 | 145,148,981 | AAACTCACCACACCTCTCCTCCCTGCAT |
| TDR | 145,148,911 | 145,148,931 | GCTGAGCTGTTGCTATCCTTG |
PCR Primers
| Primer | Sequence |
| SU | TGCACAGAGCCAGGTCTTC |
| SD | GCTGACACCCTTGACTCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15397A-H10 | VGB6 | C57BL/6NTac | |
| 15397C-F11 | VGB6 | C57BL/6NTac | |
| 15397B-G2 | VGB6 | C57BL/6NTac | |
