| Gene | Slc13a1 |
| Chromosome | 6 |
| Deletion Start | 24,168,061 |
| Deletion End | 24,137,053 |
| Deletion Size | 31,009 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,167,160 | 24,167,180 | GTGGTCTGAACAAGCAAACAC |
| TUP | 24,167,131 | 24,167,159 | TCAGTGAGCGCCTTTGTATTAGGACTGTA |
| TUR | 24,167,110 | 24,167,130 | GCTATCACCAGCTCACACTTC |
| TDF | 24,137,268 | 24,137,287 | TGCACAGTTGAAGGTCACAG |
| TDP | 24,137,238 | 24,137,262 | AGTAGTAGCCTAAGAGTGTCAGTTG |
| TDR | 24,137,141 | 24,137,164 | TCCAATTAGCAGAAGGTGAAAGTC |
PCR Primers
| Primer | Sequence |
| SU | CCTTCTCCTGGTCTTTCAAGG |
| SD | GATGGAATTGAAATCTACTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 147 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15400A-D12 | VGB6 | C57BL/6NTac | |
| 15400B-B3 | VGB6 | C57BL/6NTac | |
| 15400B-C4 | VGB6 | C57BL/6NTac | |
| 15400B-H4 | VGB6 | C57BL/6NTac | |
