| Gene | Parp12 |
| Chromosome | 6 |
| Deletion Start | 39,118,156 |
| Deletion End | 39,087,272 |
| Deletion Size | 30,885 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 39,117,836 | 39,117,859 | CTGCATCTCTGCAAGTTTCTCATC |
| TUP | 39,117,809 | 39,117,834 | ACGGCAACTGCAAGTTCCTGAAGACC |
| TUR | 39,117,788 | 39,117,806 | CCACCGTTGGCGTCCTTAC |
| TDF | 39,087,681 | 39,087,703 | GCATCACCCATTGCTAGGATGTG |
| TDP | 39,087,658 | 39,087,679 | TCTTCCAGTGCCCTGTGCAGCT |
| TDR | 39,087,635 | 39,087,656 | AGCTCCCTGAAACGATACAGAC |
PCR Primers
| Primer | Sequence |
| SU | CACGACTCCCTCAGACGC |
| SD | ACTTAGGCTCACAATCAATACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15401A-D1 | VGB6 | C57BL/6NTac | |
| 15401A-F10 | VGB6 | C57BL/6NTac | |
| 15401A-A7 | VGB6 | C57BL/6NTac | |
| 15401A-G8 | VGB6 | C57BL/6NTac | |
