| Gene | Prss58 |
| Chromosome | 6 |
| Deletion Start | 40,900,090 |
| Deletion End | 40,895,332 |
| Deletion Size | 4,759 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,899,580 | 40,899,604 | CCTACGTCCAATCACATCTCACTAC |
| TUP | 40,899,537 | 40,899,563 | ACTCCAAACCAGCATCTCTTCACTGGA |
| TUR | 40,899,502 | 40,899,525 | GAAATGGAGGGAACTATTGGCAAG |
| TDF | 40,895,529 | 40,895,550 | CTGTTGCCTGAAAGATGACCTG |
| TDP | 40,895,497 | 40,895,522 | TTTCTCCATAGGAAGTCACGGCTGCC |
| TDR | 40,895,442 | 40,895,464 | CACAGCCATCTGCATAAGACAAG |
PCR Primers
| Primer | Sequence |
| SU | CAAGTCAAAGAGCAGGAGGC |
| SD | TCAGTCAACATCTTCCCAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15403B-E2 | VGB6 | C57BL/6NTac | |
| 15403C-D4 | VGB6 | C57BL/6NTac | |
| 15403C-H6 | VGB6 | C57BL/6NTac | |
