| Gene | Gstk1 |
| Chromosome | 6 |
| Deletion Start | 42,250,353 |
| Deletion End | 42,246,025 |
| Deletion Size | 4,329 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,246,552 | 42,246,573 | CCTATGCAGATACCAACACCTC |
| TUP | 42,246,579 | 42,246,602 | TATAAAGCTGCAGTTGCGGCCCAC |
| TUR | 42,246,621 | 42,246,642 | TTCATGCTTACCGCTGTCTTTC |
| TDF | 42,249,702 | 42,249,722 | TGCCACATAGGTAGAGCTGAG |
| TDP | 42,249,728 | 42,249,753 | TCCTGAGGCCAATGAGTCTCTTAGCA |
| TDR | 42,249,779 | 42,249,801 | GCAAGTGATGATGGTCTGAGGTG |
PCR Primers
| Primer | Sequence |
| SU | TTCGGAACTTCCATATTGCC |
| SD | CTGGCTGGTCAGAGACAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15404A-G3 | VGB6 | C57BL/6NTac | |
| 15404A-G6 | VGB6 | C57BL/6NTac | |
