| Gene | Zyx |
| Chromosome | 6 |
| Deletion Start | 42,357,958 |
| Deletion End | 42,350,272 |
| Deletion Size | 7,687 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,350,699 | 42,350,717 | GGAATGTGGGCCAGGAATC |
| TUP | 42,350,721 | 42,350,750 | CCCTCTGGATCCGTTCTCTGAAGTGTAACG |
| TUR | 42,350,779 | 42,350,799 | CGAAGCAGGTCGAGTGAGAAC |
| TDF | 42,357,322 | 42,357,339 | TCCAAGGTGCTCCGTCTG |
| TDP | 42,357,342 | 42,357,363 | CGGAGCCAATCATGCCTGAGCC |
| TDR | 42,357,384 | 42,357,404 | TCTTATCCAGCGCCACTACTC |
PCR Primers
| Primer | Sequence |
| SU | TTGGCTAGAAGCGGCTCCG |
| SD | TACCCTCAGCAAGCACATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15405A-B2 | VGB6 | C57BL/6NTac | |
| 15405A-B10 | VGB6 | C57BL/6NTac | |
| 15405A-H10 | VGB6 | C57BL/6NTac | |
| 15405A-E1 | VGB6 | C57BL/6NTac | |
