| Gene | Fam115c |
| Chromosome | 6 |
| Deletion Start | 42,643,088 |
| Deletion End | 42,624,299 |
| Deletion Size | 18,790 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,642,690 | 42,642,708 | TGATGCCTACAACGACACC |
| TUP | 42,642,659 | 42,642,686 | ACCAAGAAGCTGGTCCAGTTTGTGAAGC |
| TUR | 42,642,641 | 42,642,658 | TGAGCAAGCCTCCACCAC |
| TDF | 42,624,838 | 42,624,859 | GGGTTCCAGAGAACACCTTTAC |
| TDP | 42,624,803 | 42,624,830 | AGGGATAAAGGTTGATGGCCAAGCGCTA |
| TDR | 42,624,746 | 42,624,764 | GTTTGCCCAATCCCTGGTC |
PCR Primers
| Primer | Sequence |
| SU | CCCACACCATTTCAGGTTTC |
| SD | CGCTGTATGCAGGTCTTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15406A-C8 | VGB6 | C57BL/6NTac | |
| 15406A-E6 | VGB6 | C57BL/6NTac | |
