| Gene | Hoxa6 |
| Chromosome | 6 |
| Deletion Start | 52,208,621 |
| Deletion End | 52,206,335 |
| Deletion Size | 2,287 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,208,430 | 52,208,451 | CCAACAGTCCAACTCGGTCTTG |
| TUP | 52,208,406 | 52,208,428 | CCTGCAACCGGGCATCCTACGAG |
| TUR | 52,208,340 | 52,208,361 | CTTGTTATTGCCCGAGGGTGAG |
| TDF | 52,206,716 | 52,206,736 | TGAGAAGCTGGAGTGAAGCAG |
| TDP | 52,206,677 | 52,206,706 | CAGTTTAGAGACTAATGGCTGGGAGGCACA |
| TDR | 52,206,601 | 52,206,619 | GCCCGTGACTCCCATACAC |
PCR Primers
| Primer | Sequence |
| SU | TTGTCAGGTTTCCTGTTTGG |
| SD | TTAGAATTTGATGGCGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 136 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15408A-B9 | VGB6 | C57BL/6NTac | |
| 15408A-D4 | VGB6 | C57BL/6NTac | |
| 15408A-F4 | VGB6 | C57BL/6NTac | |
