| Gene | Il12rb2 |
| Chromosome | 6 |
| Deletion Start | 67,361,947 |
| Deletion End | 67,351,243 |
| Deletion Size | 10,705 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,361,598 | 67,361,619 | AGAACCAGAAGATGCCTAGCAG |
| TUP | 67,361,567 | 67,361,595 | AAAGGCACTTGCCATAGAAGTCTAACCAC |
| TUR | 67,361,506 | 67,361,525 | CACCTGTGCCACAGAACTTG |
| TDF | 67,352,216 | 67,352,237 | GTACCCATGCTATGCGATTGTC |
| TDP | 67,352,182 | 67,352,208 | TTTAATGCCAGTTCCCACTCCTGCACA |
| TDR | 67,352,155 | 67,352,179 | TGAGAGTTTGTATGTGGATCTTGTG |
PCR Primers
| Primer | Sequence |
| SU | AGCCTGAACACAATGAGATAC |
| SD | GTGGTGACTTGACTTATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15411B-D1 | VGB6 | C57BL/6NTac | |
| 15411B-F11 | VGB6 | C57BL/6NTac | |
| 15411B-F10 | VGB6 | C57BL/6NTac | |
| 15411B-D2 | VGB6 | C57BL/6NTac | |
| 15411C-B10 | VGB6 | C57BL/6NTac | |
