| Gene | Tacr1 |
| Chromosome | 6 |
| Deletion Start | 82,403,993 |
| Deletion End | 82,403,613 |
| Deletion Size | 381 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 82,403,668 | 82,403,691 | CTGAGTCTAACCAGTTTGTGCAAC |
| TUP | 82,403,694 | 82,403,717 | ACCTGGCAAATTGTCCTTTGGGCA |
| TUR | 82,403,732 | 82,403,752 | ACCACGGAAGTCACCACGATG |
| TDF | 82,403,845 | 82,403,866 | CCTGCATGGCTGCATTCAATAC |
| TDP | 82,403,869 | 82,403,893 | TGGTGAACTTCACCTACGCAGTCCA |
| TDR | 82,403,894 | 82,403,914 | AGGCCGTAGTACCACACGTTG |
PCR Primers
| Primer | Sequence |
| SU | ACGCATGTTAACGGGAGTTG |
| SD | TCTCCCATCCAGGCACTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15412B-B8 | VGB6 | C57BL/6NTac | |
| 15412B-C3 | VGB6 | C57BL/6NTac | |
| 15412C-E5 | VGB6 | C57BL/6NTac | |
| 15412C-H9 | VGB6 | C57BL/6NTac | |
