| Gene | Rab11fip5 |
| Chromosome | 6 |
| Deletion Start | 85,374,525 |
| Deletion End | 85,336,963 |
| Deletion Size | 37,563 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,373,884 | 85,373,905 | GCCTGATGGAGTTGGTGTAGTG |
| TUP | 85,373,859 | 85,373,881 | CAAGCGTTGGCAGTACATGGCCA |
| TUR | 85,373,824 | 85,373,842 | TCCCACAGCCTCAGAATCC |
| TDF | 85,337,847 | 85,337,869 | GGGACACTAGCTGTGTAGAAGAC |
| TDP | 85,337,820 | 85,337,842 | CACGGGAAATGGCTGCCTAAGCC |
| TDR | 85,337,761 | 85,337,779 | GCCCTCCTCAATGCACCAG |
PCR Primers
| Primer | Sequence |
| SU | TTTCTTCCTTTGCAGTTCCG |
| SD | AAGTGTAGGTGCCCTGGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15413A-A4 | VGB6 | C57BL/6NTac | |
| 15413A-A12 | VGB6 | C57BL/6NTac | |
| 15413A-F12 | VGB6 | C57BL/6NTac | |
| 15413A-H8 | VGB6 | C57BL/6NTac | |
