| Gene | Fbxo41 |
| Chromosome | 6 |
| Deletion Start | 85,484,721 |
| Deletion End | 85,472,988 |
| Deletion Size | 11,734 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,483,873 | 85,483,892 | TGGAACTGCTGGCTTCACTC |
| TUP | 85,483,844 | 85,483,869 | CAGGACCTGGTGCATAAGGAACAGGA |
| TUR | 85,483,807 | 85,483,825 | GCCTCCAGGGTCTACTCAC |
| TDF | 85,473,327 | 85,473,346 | CAAGCACTGTCTCCCTTACC |
| TDP | 85,473,301 | 85,473,321 | CCCAGGCATCTGCCGGAACCT |
| TDR | 85,473,271 | 85,473,290 | GCAATCCCAATCTGCACCAC |
PCR Primers
| Primer | Sequence |
| SU | ATCCACCTAGACCTAGTAATC |
| SD | GGGAGAAGAGTCCTCCTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15414A-D5 | VGB6 | C57BL/6NTac | |
| 15414A-F7 | VGB6 | C57BL/6NTac | |
| 15414A-E4 | VGB6 | C57BL/6NTac | |
| 15414A-F3 | VGB6 | C57BL/6NTac | |
| 15414A-H12 | VGB6 | C57BL/6NTac | |
