| Gene | Egr4 |
| Chromosome | 6 |
| Deletion Start | 85,513,394 |
| Deletion End | 85,511,559 |
| Deletion Size | 1,836 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,512,809 | 85,512,830 | AGCTTCTTCATCCAGGCGGTTC |
| TUP | 85,512,787 | 85,512,805 | AACACCCGCACGACCCGGA |
| TUR | 85,512,750 | 85,512,768 | CAGGCCCAAGATGCCAGAC |
| TDF | 85,512,363 | 85,512,384 | AGTAGGGACCAAGGTCGAGGAC |
| TDP | 85,512,336 | 85,512,355 | CCATCAGCTGCCCCGCCGAG |
| TDR | 85,512,275 | 85,512,293 | GGGCCAACGAGAAGGTGTC |
PCR Primers
| Primer | Sequence |
| SU | GCATACATCCTGTGTCCATG |
| SD | TGCTTGAGAGTAGCGTCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15415A-H10 | VGB6 | C57BL/6NTac | |
| 15415A-F12 | VGB6 | C57BL/6NTac | |
