| Gene | Kcna1 |
| Chromosome | 6 |
| Deletion Start | 126,643,352 |
| Deletion End | 126,641,803 |
| Deletion Size | 1,550 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,642,807 | 126,642,831 | GGTCATCCTCATCTCCATAGTCATC |
| TUP | 126,642,782 | 126,642,804 | TTGCCTGGAGACTCTCCCTGAGC |
| TUR | 126,642,755 | 126,642,773 | TGCCCGTGAAGTCCTTGTC |
| TDF | 126,642,454 | 126,642,474 | CCGCTTGGTAAGGGTGTTCAG |
| TDP | 126,642,425 | 126,642,450 | CTTCAAACTCTCCCGCCACTCCAAGG |
| TDR | 126,642,371 | 126,642,391 | GCAGCCCTAACTCCCTCATAC |
PCR Primers
| Primer | Sequence |
| SU | CTGCACCGTGTCCTAGAAGG |
| SD | GGAAATACTTCAGGACAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15423A-B11 | VGB6 | C57BL/6NTac | |
| 15423A-G5 | VGB6 | C57BL/6NTac | |
| 15423A-G10 | VGB6 | C57BL/6NTac | |
| 15423A-H3 | VGB6 | C57BL/6NTac | |
| 15423A-H12 | VGB6 | C57BL/6NTac | |
