| Gene | Efcab4b |
| Chromosome | 6 |
| Deletion Start | 127,629,928 |
| Deletion End | 127,603,866 |
| Deletion Size | 26,063 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,604,215 | 127,604,233 | CAGTCCTTGCCTGCTGTTG |
| TUP | 127,604,238 | 127,604,265 | CTTTGCCTTCCCTTACTCTGTGACTCCC |
| TUR | 127,604,276 | 127,604,295 | GTGTTGCTGGGTCCTTTGTG |
| TDF | 127,629,815 | 127,629,838 | TCAATCAGAAGCAGAGGAAGGTAG |
| TDP | 127,629,841 | 127,629,864 | TACTGTGGAGACATCGTGGGCCCT |
| TDR | 127,629,868 | 127,629,889 | GAGGCAAGCTGAGTTGGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | TCCGTGTGTTTCTTCCCTTC |
| SD | GTTGGGTGATTCATAGGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15424A-C4 | VGB6 | C57BL/6NTac | |
| 15424A-G10 | VGB6 | C57BL/6NTac | |
