| Gene | 2900092C05Rik |
| Chromosome | 7 |
| Deletion Start | 12,515,493 |
| Deletion End | 12,512,582 |
| Deletion Size | 2,912 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,512,810 | 12,512,834 | TTCAAGGGAGTAGACAGTTAGAAAC |
| TUP | 12,512,839 | 12,512,865 | AAATCTTCTATGGGACAGCCCAGAGGC |
| TUR | 12,512,885 | 12,512,909 | CGTGCTGGGATTATTACTTCTGTTG |
| TDF | 12,514,560 | 12,514,579 | GGCTCGCCAACCAGTCTAAG |
| TDP | 12,514,581 | 12,514,604 | CAAGGCCACGGAAGCTGCAAGCTG |
| TDR | 12,514,605 | 12,514,628 | GAACTGCTTGTGAGGTTCCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTTCTCCTCACATGCCACAC |
| SD | CAGAAAGTCAGCACCAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15433A-E4 | VGB6 | C57BL/6NTac | |
| 15433B-A2 | VGB6 | C57BL/6NTac | |
| 15433C-F2 | VGB6 | C57BL/6NTac | |
| 15433C-G11 | VGB6 | C57BL/6NTac | |
| 15433C-A1 | VGB6 | C57BL/6NTac | |
