| Gene | Grik5 |
| Chromosome | 7 |
| Deletion Start | 25,068,519 |
| Deletion End | 25,046,179 |
| Deletion Size | 22,341 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,067,746 | 25,067,765 | ACGTACAGTCCCAGATCACC |
| TUP | 25,067,725 | 25,067,744 | TGCAGAGCAGGCTTGCCCTG |
| TUR | 25,067,688 | 25,067,707 | TGAGCATCGACAGACACTTC |
| TDF | 25,046,971 | 25,046,990 | GGTTCAGTCTGGGTGCTGTG |
| TDP | 25,046,948 | 25,046,970 | AGACCCAGTCCAGAGGCAGCTCA |
| TDR | 25,046,912 | 25,046,935 | TCCCAGGCACTAGAATTACAAGTC |
PCR Primers
| Primer | Sequence |
| SU | CTAGTAACAGCTGCCTGCCC |
| SD | CAGACAGCAGTCCTTGCTCT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15443A-E2 | VGB6 | C57BL/6NTac | |
| 15443A-G10 | VGB6 | C57BL/6NTac | |
| 15443A-E6 | VGB6 | C57BL/6NTac | |
| 15443A-A8 | VGB6 | C57BL/6NTac | |
