| Gene | Cpt1c |
| Chromosome | 7 |
| Deletion Start | 44,973,864 |
| Deletion End | 44,959,475 |
| Deletion Size | 14,390 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,973,184 | 44,973,205 | CCCACATCCGAGACCCTAGAAG |
| TUP | 44,973,156 | 44,973,182 | TCCCAAGAGTGAGCCTCTCCTTTCTCA |
| TUR | 44,973,123 | 44,973,144 | AGGGACCACACCAGCAAGAAAG |
| TDF | 44,959,681 | 44,959,700 | TAGCGCCTTATCTGCTTCTC |
| TDP | 44,959,660 | 44,959,680 | TGCAGGACTCTCACCGGCTGG |
| TDR | 44,959,614 | 44,959,633 | CGGAACAGGGAGGCTACATC |
PCR Primers
| Primer | Sequence |
| SU | TCTCCAACTGTGAGCTGCAG |
| SD | TTGCTGTCCAGACCTCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15451A-G9 | VGB6 | C57BL/6NTac | |
| 15451A-A12 | VGB6 | C57BL/6NTac | |
