| Gene | Ldhc |
| Chromosome | 7 |
| Deletion Start | 46,877,886 |
| Deletion End | 46,861,773 |
| Deletion Size | 16,114 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,861,979 | 46,861,999 | GTTGGCTACGTGTGGTCATTG |
| TUP | 46,862,003 | 46,862,029 | CGGGCCTGTCATCTTAGGACTTGGTAA |
| TUR | 46,862,075 | 46,862,095 | CAGCCCAGCCTCAAAGTTATC |
| TDF | 46,876,995 | 46,877,016 | CACGTATAGGTTGCCCAGATTC |
| TDP | 46,877,020 | 46,877,047 | CAGCTGTTTCTGTAGCCTTGGCTTTGGT |
| TDR | 46,877,062 | 46,877,080 | GTGCCAGTAGCCCACATTC |
PCR Primers
| Primer | Sequence |
| SU | AAAGCCATTGTAGGCCTGTG |
| SD | AATGGAATTTCACCCTGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15453A-A7 | VGB6 | C57BL/6NTac | |
| 15453A-A11 | VGB6 | C57BL/6NTac | |
| 15453A-G6 | VGB6 | C57BL/6NTac | |
| 15453A-G4 | VGB6 | C57BL/6NTac | |
| 15453A-D11 | VGB6 | C57BL/6NTac | |
