| Gene | Gabra5 |
| Chromosome | 7 |
| Deletion Start | 57,508,061 |
| Deletion End | 57,488,807 |
| Deletion Size | 19,255 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,507,304 | 57,507,324 | CAGAAGGCTTGCAGGTTGAAG |
| TUP | 57,507,279 | 57,507,302 | ACTCTCACTGCACAGAGTGTGCCC |
| TUR | 57,507,234 | 57,507,256 | CGAAGTGTTCCCTTGGGAAAGAG |
| TDF | 57,489,231 | 57,489,253 | CACAGTATAGAGGAGGCTCTTGG |
| TDP | 57,489,202 | 57,489,229 | AAGGCAGTGCAAAGCAACCTATGTCTGT |
| TDR | 57,489,173 | 57,489,191 | GTTCGCACCTGCGTGATTC |
PCR Primers
| Primer | Sequence |
| SU | TTGGGTCATTTATACCCTGC |
| SD | CAACTTCAAAGGATGCTCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15455A-B3 | VGB6 | C57BL/6NTac | |
| 15455A-E11 | VGB6 | C57BL/6NTac | |
| 15455A-G5 | VGB6 | C57BL/6NTac | |
